Category Archives: Genomics & more

A short list of tools for shotgun metagenomics

We are currently finalizing our work on the analysis of metagenomes for soils taken from the Etosha National Park, Namibia. These soils were interesting since Zebra blood containing the nasty bug Bacillus anthracis had poured into it. Our aim was … Continue reading

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Removing small & low coverage contigs from a Spades assembly

At the moment I am processing quite a few bacterial genome assemblies that I created for various Thermotogae species. I am comparing assemblies created by CLC, Velvet and the latest SPAdes assembler with REAPR. Both CLC and Velvet were set … Continue reading

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Installing phiSpy on my macbook.

The reason for this post is that in my current project I need to identify prophages in Thermotogales genomes. Prophages are phages /virusses that are integrated into the genome of their host, in our case Bacteria. To check if our … Continue reading

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Is improvement possible? Quality control of a Illumina Nextera dataset for the novo genome assembly

In my current postdoc I work on genome analysis of multiple thermophilic bacteria belonging to the phylum Thermotogae. The data for such analyses comes from DNA shotgun sequencing. A technique were DNA chains are broken up in many small pieces … Continue reading

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