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Tag Archives: sequencing
How many PCR cycles should I use to create an amplicon library?
One of my side projects deals with the gut microbiota of Atlantic Cod (Gadus morhua). In 2014 we published a pilot experiment on this where we sequenced the 16S rRNA to get a taxonomical overview of the microbial gut content … Continue reading
Is improvement possible? Quality control of a Illumina Nextera dataset for the novo genome assembly
In my current postdoc I work on genome analysis of multiple thermophilic bacteria belonging to the phylum Thermotogae. The data for such analyses comes from DNA shotgun sequencing. A technique were DNA chains are broken up in many small pieces … Continue reading
Posted in Genomics & more, High-throughput Sequencing
Tagged assembly, bacteria, bad data, CLC assembler, E. coli, fastqc, genome, high-throughput, Illumina, MiSeq, paired-end, preqc, Quality control, Rubbish, sequencing, Thermotoga maritima, Thermotogae
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The Microsized Mind 